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More Great Information on Straight Ebony:
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Biology- Testcrossing Question?
Any help you can offer me on this problem is greatly appreciated 
!
A genetics student mated Drosophila females that were homozygous for the recessive genes cu (curled), sr (striped), and e (ebony) with males that were homozygous for the wild type: cu+ (straight), sr+ (not stripled), and e+ (gray). She then testcrossed the F1 females with homozygous recessive curled, striped, ebony males.
Results:
straight, gray, not striped 820
curled, ebony, striped 810
straight, ebony, striped 100
curled, gray, not striped 97
straight, ebony, not striped 80
curled, gray, striped 90
straight, gray, striped 1
curled, ebony, not striped 2
How are the 3 genes arranged on the chromosomes? How can you tell? If any of the genes are linked, how far apart are they on the chromosome? How can you determine this?
Thanks!
The three genes are arranged in the order curled -- ebony -- striped. We can map the genes using recombinant frequencies. From the phenotypes, we know that straight-gray-not striped and curled-ebony-striped are parental phenotypes, and thus there were no crossovers. The next two had a crossover between the curled and the ebony genes, the next two are a double crossover, and the last two had a crossover between the ebony and striped genes.
Because there are three genes, we must calculate the distance between each pair of genes. You do this by adding up the recominants for each pair. For example, for the pair curly-ebony, straight-ebony-striped, curled-gray-not striped, straight-ebony-not striped, and curled-gray-striped are recombinant. Adding up their frequencies gives you 367/2000 or 18.35 %.
Once this is done for all three pairs, we get RFs of 8.65% between ebony-striped, and 10% between curly-striped. When mapping this out, however, the numbers do not add up. We must correct for the double crossover -- the 5th and 6th phenotypes on your list. To correct, we double the number and add these to the other recombinants for cu-sr. We now get an RF of 540/2000 or 27%.
Now, when we map it out we get:
cu-e 18.35 mu
e-sr 8.65 mu
cu-sr 27.00 mu
These numbers add up.
If you would like a more detailed explanation including how I set up my charts, please ask.