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More Great Information on Pairs Circular:
How Much For Judy Garland's 'Wizard Of Oz' Ruby Slippers? (AOL Television)
On the season premiere of "Hollywood Treasure" (Tue., 10 p.m. ET on Syfy) auctioneer Joe Maddalena got his hands on a legendary piece of Hollywood history: Judy Garland's ruby slippers from "The Wizard of Oz." Although four pairs of the slippers are known to exist, these were the actual ones that Garland clicked her heels in during the "there's no place like home" scene at the end of the movie. The slippers had "Judy Garland" written in the lining and, most excitingly of all, "what appear to be very slight circular scuff marks. And that would of course be from when Judy -- clicking her heels." How much for the iconic footwear? A whopping $2 million. _See more treasure troves uncovered on "Hollywood Treasure," Tuesdays at 10 p.m. ET on Syfy._ _TV Replay scours the vast television landscape to find the most interesting, amusing, and, on a good day, amazing moments, and delivers them right to your browser._
Please help on a Multiple Choice Question about DNA Synthesis?What will happen when the two hydrogen-bonding strands of a 5000 base pair circular DNA plasmid are dissociated over a continuous length of 50 base pairs without cleaving the phosphodiester backbone? A. Nothing, the DNA will remain unaffected B. The writhing and linking number will decrease C. The writhing number will increase and the twist will decrease D. The twist will increase and the Writhe will decrease E. The DNA will untwist and the writhing number will remain constant. My guess is C because if you unwide a DNA strand without cleaving any bonds, W usually increases if W increases, T must decrease to keep L constant since no cleaving occured.
C. Since the linking number L of supercoiled DNA is the number of times the two strands are intertwined (and both strands remain covalently intact), L cannot change. L = T + W This eliminates answers A, B and E. So the question then becomes whether the twist or the writhe are increasing. If the twist increases then the writhe must decrease since L remains constant (Answer D). But if the write increases the twist must decrease (Answer C). The twist is the number of helical turns in the DNA and the writhe is the number of times the double helix crosses over on itself (these are the supercoils). Dissociating the two strands of a circular DNA plasmid wil result in decreasing the number of helical turns (i.e., the DNA is being linearized in that region). Therefore the answer is C.